When: Thursday, February 20, 2025 2:00 PM – 3:30 PM

International Barley Hub is pleased to announce the next in the 2025 series of seminars: “A barley pan-transcriptome reveals layers of genotype-dependent transcriptional complexity” Presented by Miriam Schreiber, The James Hutton Institute & Vana Marosi, Helmholtz Munich

Summary:

Barley genomic resources provide insights into barley genetics and diversity. One new resource is a barley pan-transcriptome (https://doi.org/10.1038/s41588-024-02069-y) which captures the transcriptional and post-transcriptional consequences of barley pan-genome diversity. To avoid issues associated with single reference bias, we developed multiple genotype-specific reference transcript datasets (RTDs) and integrated them into a single pan-RTD. The pan-RTD allowed us to study differences in transcript abundance between both tissues and genotypes and to explore the origin of some of these differences. Here we will present this valuable resource and show how it can be used by the community to study the expression of candidate genes from a multiple-genotype perspective. 

Speakers bio:

Miriam Schreiber:

Miriam Schreiber is a cereal bioinformatics specialist at the James Hutton Institute and the International Barley Hub in Dundee, Scotland, UK. Her work focuses mostly on barley, but sometimes other crops slip in. She did her undergrad in Aachen, Germany before joining the University of Dundee for her PhD. Miriam has since worked in Dundee on different projects. For example, the ERC Meiosis shuffle project which identified EMS induced variants in meiotic genes in the cultivar Golden Promise, or the BARN project which focused on big transcriptome and genotype datasets of a barley European spring two-row collection. Recently she has embraced the pan world (pan-genome, pan-transcriptome) with her main focus on mining those big datasets for interesting stories. 

Vanda Marosi:

Vanda Marosi is a final-year doctoral candidate at Helmholtz Munich, Germany, in the group of Prof. Klaus F.X. Mayer. She completed her master’s degree in molecular biology at Ludwig-Maximilians University of Munich and pursued her PhD in computational biology. Her research focuses on comparative transcriptomics, investigating ortholog diversification and diversity of gene expression patterns in cereals, particularly barley and wheat. She has developed a comprehensive pipeline to compare gene co-expression networks across different species and cultivars. Recently, Vanda joined Prof. Nadia Kamal’s group at the Technical University of Munich as a postdoctoral researcher within the ERC project “RESIST”, where she will expand her analysis to oats.