High throughput measuring of meiotic recombination rates in barley pollen nuclei using Crystal Digital PCRTM

Ahn et al (2021) Pre-publication 

The paper explores the use of a Crystal Digital PCRTM‐based genotyping assay to measure recombination in individual Flow sorted barley pollen prior to fertilization.  This allows recombination patterns to be assessed without the need to develop asegregating population. The authors have shown that the method is reliable in the measurement of recombination in hybrid plants, providing a novel cost-effective way to select for new allelic combinations during plant breeding.